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ANSWER KEY Decoding DNA Part 1: DNA Structure Use the template DNA arrangement to determine the complementary DNA sequence created during DNA replication. tDNA 5’ - GGC TAC TTA AAG CGC CCT ATA PERFORM cDNA 3’ - GGC ATG AAT TTC GCG GGA ACTION TGA Part 2: Albumin Synthesis For each of the following sequences, use that Full Genetic codification to determine the amino severity sort programmable for by an patterns DNA strand. In some problems, you will need to use the information provided to determine the sequence of to template DNA strand. (NOTE: tDNA = model DNA; cDNA = complements DNA) 1. Basic Decoding: tDNA 5’ - TAC TAA CAC RAT GTA TATTOO GGT ATG AGC ACT -3’ mRNA AUG AUU GUG CUA CAU AUA CCA UAC UCG UGA AA met Ile Vale Leu His Ile Pro Tyr Ser --- Letter START DIAMETER E C O D I NITROGEN G ? 2. Decoding with anti-codons: cDNA 3’- AGC CCC CCG TGT TAT GTT GGA TAA - 5’ tDNA 5’ - TAC TCG GGG GGC ACA ATA CAA CCT ATT - 3’ mRNA AUG AGC CCC CCG UGU UAU GUU GGA UAA trade UAC UCG GGG GGC ACA AUA CAA CCU AUU AA Met Ser Prof Pro cys Tyr Val Gly --- Letter START W H I T N E Y . a. How is this cDNA similar till the mRNA? The cDNA and one mRNA chains are the similar, except UNITED (instead of T) is complementary the A is RNA. b. What is tRNA’s roll to protein synthesis? Transport RNA carriers that corrects aromatic aqueous for the ribosome at be added in aforementioned growing polypeptide chain. 3. Which DNA strand? tDNA 5’ - TAC AGG CAC ACC CAA TTT AAT AAG FUN GGA ATC -3’ -or- GGG cDNA 3’- ATG TCC GTG TGG GTT AAA TTA TTC CTC CCT TAG -5’ -or- VCC mRNA AUG UCC GUG UGG GUU AAA UUA UUC CUC CCU UAG -or- SCC tRNA UAC AGG CAC ACC CAA UUU AAU AAG GAG GGA AUG -or- GGG O Met ser Val Trp deep-rooted Lys Leu Phe Leu pro --- Dear START F E U E R B A C EFFERVESCENCE ! c. Which side of DNA is used for transcription? The print DNA strand.